Hydrolyzed Sodium Hyaluronate Powder — Enzymatic mini HA 3-5 kDa Supplier
Hydrolyzed Sodium Hyaluronate Enzymatic mini HA (INCI: Hydrolyzed Sodium Hyaluronate, CAS 9067-32-7, MW 3-5 kDa) — the smallest commercially available HA grade, produced via proprietary controlled enzymatic hydrolysis. At 3-5 kDa (~15-25 disaccharide units), these ultra-short HA fragments achieve maximum dermal penetration — traversing the full epidermal thickness, crossing the basement membrane, and reaching the deepest reticular dermis where fibroblasts, collagen, and elastin reside. The proprietary hyaluronidase enzyme technology cleaves at specific glycosidic bonds under precisely controlled conditions (pH 5.0-6.0, 37°C, timed reaction) — producing a narrow, uniform MW distribution (PDI <1.3, GPC-MALLS) with preserved native HA structure and full bioactivity, unlike chemical hydrolysis which randomly cleaves chains and can destroy bioactive signaling motifs. At this MW, HA fragments function as potent bioactive signaling molecules: CD44v6/v9 receptor binding (Kd ~10-50 nM) activates MAPK/ERK fibroblast proliferation, RHAMR-FAK keratinocyte migration, and VEGF-R2 angiogenesis — delivering multi-target anti-aging at the deepest dermal level. COSMOS-certified, ISO 9001:2015, c-GMP, HALAL, KOSHER. Enzymatic mini HA manufacturer and bulk supplier — UPOR Biotech.
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Hydrolyzed Sodium Hyaluronate Enzymatic mini HA (INCI: Hydrolyzed Sodium Hyaluronate, CAS 9067-32-7, MW 3-5 kDa, ~15-25 disaccharide units) is the smallest commercially available HA grade — produced through proprietary controlled enzymatic hydrolysis using specific hyaluronidase (HYAL1/HYAL2) enzymes under precisely optimized conditions. At 3-5 kDa, this is approximately half the molecular size of Oligo HA (7-10 kDa), enabling maximum dermal penetration depth — traversing the stratum corneum, viable epidermis, basement membrane, papillary dermis, and into the reticular dermis where dense collagen fiber networks, elastin, and dermal fibroblasts reside. The proprietary enzymatic hydrolysis technology provides five key advantages over chemical hydrolysis (acid/alkali/heat): (1) Structural integrity — HYAL1 enzymes cleave at the β1→4 glycosidic bond between GlcNAc and GlcA, producing fragments with native reducing-end GlcNAc and non-reducing-end GlcA — the exact termini recognized by cell-surface CD44 and RHAMM receptors. Chemical hydrolysis randomly cleaves at multiple sites, generating heterogeneous termini with reduced receptor affinity. (2) Uniform MW distribution — controlled enzyme:substrate ratio, pH 5.0-6.0 (HYAL1 pH optimum), 37°C, and timed reaction quenching produce a narrow, consistent 3-5 kDa distribution (PDI <1.3, GPC-MALLS verified). Chemical hydrolysis yields broad, poorly reproducible MW distributions. (3) Higher purity — enzymes are completely removed post-reaction by ultrafiltration (3 kDa MWCO membrane) and ion-exchange chromatography; no residual acids, alkalis, or organic solvents. (4) Bioactivity preservation — the gentle enzymatic process (37°C, neutral pH) maintains CD44v6/v9 and RHAMR binding motifs; chemical hydrolysis (80-100°C, pH 1-2 or pH 12-13) can denature or destroy these motifs. (5) Green chemistry — enzyme-based production is environmentally sustainable with lower energy requirements and zero hazardous chemical waste. At 3-5 kDa, these HA fragments are at the optimal size for dual-function bioactivity + deep penetration: small enough to reach the reticular dermis, large enough to maintain CD44/RHAMR receptor binding (minimum ~2-3 kDa, ~10-15 disaccharide units required for bivalent receptor cross-linking and signaling activation). UPOR Biotech manufactures under c-GMP and ISO 9001:2015 with COSMOS certification.
Enzymatic vs Chemical Hydrolysis — The Proprietary Enzyme Advantage
Chemical hydrolysis uses acids, alkalis, or heat to randomly break HA chains — a crude process producing heterogeneous fragments, destroying bioactive CD44/RHAMR binding motifs, and leaving chemical residues. Proprietary enzymatic hydrolysis uses specific HYAL1 hyaluronidase enzymes that cleave at precise β1→4 glycosidic bonds under gentle conditions (pH 5.0-6.0, 37°C), producing uniform 3-5 kDa fragments with native reducing/non-reducing termini that CD44v6/v9 receptors actively recognize. The result: a cleaner product with no chemical residues, narrow MW distribution (PDI <1.3), and fully preserved bioactive signaling — delivering maximum deep dermal anti-aging efficacy at minimal concentrations with batch-to-batch reproducibility that chemical methods cannot match.
Technical Specifications
| Property | Specification |
|---|---|
| Product Name | Hydrolyzed Sodium Hyaluronate Powder — Enzymatic mini HA |
| CAS Number | 9067-32-7 |
| MW Range | 3 – 5 kDa (~15-25 disaccharide units; GPC-MALLS; PDI <1.3) |
| Production Method | Proprietary controlled enzymatic hydrolysis (HYAL1 hyaluronidase; pH 5.0-6.0, 37°C; enzyme removed by ultrafiltration + IEC) |
| Key Advantage | Smallest commercial HA — reticular dermis penetration. CD44v6/v9/RHAMR bioactive signaling with preserved native termini. Enzymatic: uniform MW, no chemical residues, fully bioactive. |
| Appearance | White to off-white powder |
| Purity (HPLC) | ≥99.0% |
| pH (0.1% w/v) | 5.5 – 7.0 |
| Solubility | Highly soluble; clear, low-viscosity solution |
| Loss on Drying | ≤10.0% |
| Protein Content | ≤0.1% |
| Heavy Metals | ≤10 ppm |
| Residual Enzyme | Not detected (HYAL1 removed by 3 kDa UF + IEC; verified by Bradford and enzyme activity assay) |
| Recommended Usage | 0.01 – 0.1% w/w (highly potent at trace levels) |
| Grade | Cosmetic Grade / Food Grade / Bioactive Grade |
| Certifications | ISO 9001:2015, c-GMP, FDA, HALAL, KOSHER, COSMOS, USP/EP |
| Packaging | 1 kg / 5 kg / 25 kg sealed containers |
| Storage | 15-25°C; dry, dark; tightly sealed; shelf life 24 months |
Key Benefits — Enzymatic mini HA
Maximum Dermal Penetration — Reticular Dermis Depth
At 3-5 kDa, penetrates to the deepest reticular dermis — beyond papillary dermis reachable by Oligo HA (7-10 kDa). Reaches dense collagen fiber networks, elastin, and deep fibroblasts. The smallest, most penetrating commercial HA grade.
Max DepthPreserved Native Bioactivity — Enzymatic Precision
Proprietary HYAL1 hydrolysis produces fragments with native GlcNAc/GlcA termini — the exact chemical structures recognized by CD44v6/v9 and RHAMR receptors. Chemical hydrolysis randomly cleaves, destroying these critical binding motifs. Full bioactive signaling preserved.
BioactiveUniform MW — Narrow Distribution for Reproducibility
Controlled enzyme:substrate ratio delivers consistent 3-5 kDa distribution (PDI <1.3, GPC-MALLS) batch-to-batch. Chemical hydrolysis yields broad, poorly reproducible distributions. Ensures predictable penetration depth and receptor activation in every formulation batch.
ReproducibleGreen Chemistry — Zero Hazardous Waste
Enzyme-based production at 37°C, neutral pH — lower energy, zero hazardous chemical waste. Enzymes removed by ultrafiltration + IEC — no residual acids, alkalis, or solvents. Environmentally sustainable manufacturing meeting green chemistry principles.
Green ChemApplications
Ultra-Premium Anti-Aging — Maximum Depth Delivery
Flagship active for luxury anti-aging creams, serums, and ampoules at 0.03-0.05%. Reticular dermis penetration targets deep wrinkles and loss of firmness at the deepest fibroblast-collagen level.
Professional-Grade Serums — High-Potency Bioactive
High-potency active for professional and medical-grade serums at 0.05-0.1%. Maximum penetration + preserved CD44/RHAMR bioactivity = clinically meaningful anti-aging at low use levels.
Aesthetic Medicine — Mesotherapy and Post-Procedure
Bioactive for mesotherapy solutions, post-procedure recovery formulations, and professional aesthetic treatments. Deep reticular dermal delivery supports fibroblast activation and collagen remodeling.
Maximum Bioavailability Supplements — Oral HA
Ultra-low MW (3-5 kDa) for enhanced GI absorption in premium beauty-from-within nutraceuticals. Smaller than Oligo HA — maximum oral bioavailability for systemic skin and joint benefits.
Regenerative Skincare — Deep Cellular Renewal
Advanced bioactive for regenerative and reparative formulations at 0.03-0.05%. CD44v6/RHAMR signaling activates fibroblast proliferation pathways for deep cellular renewal.
Scalp and Hair Care — Deep Follicular Penetration
Deep-penetrating bioactive for premium scalp serums and hair growth formulations. Reticular dermis penetration reaches the hair follicle bulb and dermal papilla for follicular health support.
MW Comparison — HA Grades
| Grade | MW | Primary Function | Penetration |
|---|---|---|---|
| High MW | 1,500-2,000 kDa | Film-forming, surface hydration | Surface only |
| Oligo HA | 7-10 kDa | Cellular signaling, wound healing | Deep dermis |
| Enzymatic mini HA (this product) | 3-5 kDa | Maximum penetration, deep anti-aging, enzymatic purity | Reticular dermis (max depth) |
Frequently Asked Questions
Enzymatic mini HA (3-5 kDa, ~15-25 disaccharide units) is the smallest commercially available HA grade, produced through proprietary controlled HYAL1 enzymatic hydrolysis. Enzymes cleave at specific β1→4 glycosidic bonds under gentle conditions (pH 5.0-6.0, 37°C), producing uniform fragments with native GlcNAc/GlcA termini that CD44v6/v9 and RHAMR receptors actively recognize. Unlike chemical hydrolysis, the enzymatic process preserves structural integrity, produces narrow MW distribution (PDI <1.3), and leaves no chemical residues.
Enzymatic mini HA (3-5 kDa) is approximately half the molecular size of Oligo HA (7-10 kDa), enabling penetration to the reticular dermis (deepest dermal layer) vs Oligo HA’s deep dermis limit. The proprietary enzymatic process produces more uniform MW distribution with preserved native receptor-binding termini — superior batch-to-batch reproducibility and bioactivity vs chemically hydrolyzed alternatives.
(1) Structural integrity — enzymatic cleavage at specific glycosidic bonds preserves native GlcNAc/GlcA termini for CD44/RHAMR recognition. (2) Uniform MW — narrow 3-5 kDa distribution (PDI <1.3) for predictable, reproducible performance. (3) Higher purity — enzymes completely removed; no residual acids/alkalis/solvents. (4) Bioactivity preservation — gentle 37°C, neutral pH maintains signaling motifs; chemical hydrolysis (80-100°C, extreme pH) destroys them. (5) Green chemistry — lower energy, zero hazardous waste.
ISO 9001:2015, c-GMP, FDA-registered, HALAL, KOSHER, COSMOS Natural Certified. Meets USP/EP. Proprietary enzyme technology with full documentation: COA (MW distribution PDI <1.3 by GPC-MALLS, purity ≥99.0%, residual enzyme not detected), MSDS, TSE/BSE, enzyme activity certificate, stability data, complete lot traceability.
0.01-0.1% w/w — highly potent at very low concentrations due to 3-5 kDa MW maximizing dermal penetration and superior enzymatic bioactivity (preserved CD44/RHAMR binding motifs). At 0.05%, significant deep dermal anti-aging. Incorporate into water phase below 40°C. Combine with higher MW HA for multi-level: surface film + deep dermal bioactive anti-aging.