6-Hydroxycarboline Derivative — Functionalized β-Carboline Scaffold, Heterocyclic Building Block, ≥98% HPLC, Research Grade Supplier
6-Hydroxycarboline Derivative (CAS 54660-75-2) is a functionalized analog of the β-carboline (9H-pyrido[3,4-b]indole) scaffold — one of the most important privileged heterocyclic frameworks in medicinal chemistry and natural product biosynthesis — bearing a hydroxyl substituent at the 6-position of the indole ring. The β-carboline core is the defining structural element of the harmala alkaloids, eudistomins, and endogenous mammalian β-carbolines, engaging diverse pharmacological targets including monoamine oxidases, serotonin receptors, cyclin-dependent kinases, and benzodiazepine binding sites. The 6-OH substituent enhances radical-scavenging antioxidant capacity, provides a versatile synthetic handle for further derivatization, and contributes critical hydrogen-bond interactions to target binding. ≥98% HPLC purity, ISO 9001:2015 and c-GMP certified. Premium research-grade supply from UPOR Biotech.
Request a QuoteProduct Overview
6-Hydroxycarboline Derivative (CAS 54660-75-2) is a functionalized heterocyclic compound built upon the β-carboline (9H-pyrido[3,4-b]indole) scaffold — a tricyclic aromatic system consisting of a pyridine ring (C-ring) ortho-fused to an indole nucleus (A+B rings). The β-carboline framework is a true privileged scaffold in the medicinal chemistry sense (as defined by Evans, 1988): a single molecular framework capable of providing high-affinity ligands for more than one distinct receptor type through appropriate substitution. The core is isoelectronic with anthracene (14 π-electrons distributed over three fused rings), producing a planar, aromatic surface that supports DNA intercalation, π-π stacking with aromatic amino acid side chains (Phe, Tyr, Trp) in protein binding pockets, and characteristic blue-green fluorescence (λex ~340-370 nm, λem ~440-480 nm). The 6-position of the indole ring is the most electronically activated site for electrophilic substitution and is conjugated with the indole nitrogen (N9) through the extended indole π-system. Introduction of a hydroxyl group at this position creates a hydrogen-bond donor/acceptor, a synthetic derivatization handle (via O-alkylation, O-acylation, or conversion to the triflate for cross-coupling), and a potent radical-scavenging antioxidant pharmacophore through the classical phenolic antioxidant mechanism (H-atom transfer to peroxyl radicals, forming a resonance-stabilized phenoxyl radical delocalized across the indole π-system). In nature, β-carboline alkaloids are derived from tryptophan (or tryptamine) via the Pictet-Spengler condensation with an aldehyde (or α-keto acid), followed by oxidative decarboxylation and aromatization. The resulting 1,2,3,4-tetrahydro-β-carboline intermediates are oxidized to the fully aromatic β-carboline (norharman, harman) or remain partially saturated (harmaline, tetrahydroharman). 6-Hydroxy-β-carboline (the unsubstituted parent, CAS 58982-28-8) is found as an endogenous mammalian metabolite and as a constituent of medicinal plants including Peganum harmala (Syrian rue) and Banisteriopsis caapi (ayahuasca vine). The derivative form (CAS 54660-75-2) features additional functionalization that tailors the scaffold’s physicochemical properties, target selectivity, and synthetic utility for specific research applications. The compound’s extended π-conjugation gives rise to characteristic UV-Vis absorption bands (λmax ~290-310 nm for the β-carboline chromophore, with a red-shifted shoulder for the 6-OH derivative due to the electron-donating mesomeric effect of the hydroxyl) and solvent-dependent fluorescence (larger Stokes shift in polar protic solvents due to excited-state H-bond reorganization), properties that have been exploited for developing fluorescent probes, pH sensors, and solvatochromic reporters based on the 6-hydroxycarboline core.
UPOR Biotech supplies the 6-Hydroxycarboline Derivative at Research Grade with a minimum assay of ≥98.0% by HPLC, manufactured under ISO 9001:2015 and c-GMP quality management systems. The material is supplied as a crystalline solid (appearance may vary from off-white to pale yellow depending on substitution pattern) with comprehensive analytical characterization including HPLC purity, ¹H/¹³C NMR, high-resolution mass spectrometry, and FT-IR. The compound is soluble in DMSO, DMF, methanol, and ethanol; solubility in aqueous buffers is limited (typical of extended aromatic heterocycles) but can be enhanced by pH adjustment (>pH 9 for phenolate formation) or by formulation with co-solvents or cyclodextrins for biological studies. The 6-OH group and the indole NH are both slightly acidic (pKa ~8.5-9.5 for 6-OH; pKa ~15-16 for indole NH), with the 6-OH being predominantly neutral at physiological pH 7.4, facilitating passive membrane permeability — a critical property for CNS-targeted β-carboline research. We serve academic and pharmaceutical researchers investigating β-carboline medicinal chemistry, indole alkaloid total synthesis, MAO enzymology, serotonin receptor pharmacology, and fluorescent probe development. Research Use Only (RUO) — not for human diagnostic or therapeutic use.
The β-Carboline Privileged Scaffold — A Single Framework Spanning Multiple Pharmacological Targets
The β-carboline scaffold — defined by the 9H-pyrido[3,4-b]indole tricyclic core — is one of the most remarkable privileged structures in all of medicinal chemistry. From a single planar, aromatic framework, appropriately substituted β-carbolines have produced high-affinity ligands for: monoamine oxidase A and B (harmine, harmaline — reversible MAO-A inhibitors with IC₅₀ values in the nanomolar range; used as antidepressants and neuroprotective agents), serotonin 5-HT₂A and 5-HT₂C receptors (6-substituted β-carbolines — psychedelic and anxiolytic pharmacophores), benzodiazepine/GABA-A receptor (β-carboline-3-carboxylate esters — inverse agonists, antagonists, and partial agonists with proconvulsant, anxiogenic, or anxiolytic profiles depending on the ester substituent), cyclin-dependent kinases (CDK1/2/5 — ATP-competitive inhibitors with antiproliferative activity), IκB kinase (IKK) (anti-inflammatory β-carbolines), topoisomerase I and II (DNA intercalating β-carbolines with anticancer activity), and imidazoline I₁/I₂ receptors (harmane, norharmane — endogenous ligands implicated in blood pressure regulation and insulin secretion). The 6-hydroxy substituent adds the phenolic antioxidant pharmacophore to this already rich target profile, creating a multi-functional molecular tool. For medicinal chemistry researchers: the 6-hydroxycarboline scaffold is a versatile starting point for focused library synthesis, structure-activity relationship studies, and the development of chemical probes targeting any of the above receptor systems.
Technical Specifications
| Property | Specification |
|---|---|
| Product Name | 6-Hydroxycarboline Derivative — Functionalized β-Carboline Scaffold, ≥98% HPLC, Research Grade |
| Synonyms / Common Names | 6-Hydroxy-β-carboline derivative; 6-Hydroxy-9H-pyrido[3,4-b]indole derivative; Functionalized 6-hydroxycarboline; 6-Hydroxynorharman derivative; 6-OH-β-carboline derivative |
| CAS Number | 54660-75-2 |
| Molecular Formula | C₁₁H₈N₂O (6-hydroxy-β-carboline core); derivative molecular formula available in CoA |
| Molecular Weight | 184.19 g/mol (6-hydroxy-β-carboline core); derivative MW available in CoA |
| Appearance | Off-white to pale yellow crystalline powder (color may vary depending on specific derivative substitution) |
| Key Feature | Functionalized β-carboline privileged scaffold with 6-OH substituent — tricyclic pyrido[3,4-b]indole heterocycle supporting π-π stacking, H-bonding, and DNA intercalation; 6-OH provides antioxidant pharmacophore, synthetic derivatization handle, and target-binding H-bond interactions |
| Scaffold Class | β-Carboline (9H-pyrido[3,4-b]indole) — privileged heterocyclic scaffold; isoelectronic with anthracene (14π aromatic system); core of harmala alkaloids, eudistomins, and endogenous mammalian β-carbolines |
| Assay (HPLC-UV) | ≥98.0% |
| Identification (¹H-NMR) | Characteristic indole NH at δ 11.0-12.0 ppm (DMSO-d₆); aromatic protons H1/H3/H4/H5/H7/H8 in the δ 7.0-9.0 ppm region; 6-OH broad singlet at δ 9.0-10.5 ppm (exchangeable with D₂O); derivative-specific signals reported on CoA |
| Identification (ESI-MS) | [M+H]⁺ consistent with expected molecular weight; exact mass reported on CoA |
| Identification (FT-IR) | Characteristic O-H stretch ~3200-3500 cm⁻¹ (broad, 6-OH), indole N-H stretch ~3400 cm⁻¹ (sharp), aromatic C=C stretches ~1600-1450 cm⁻¹, pyridine C=N stretch ~1620 cm⁻¹ |
| Melting Point | Reported on Certificate of Analysis (varies with derivative structure; typically >180°C for β-carboline class) |
| UV-Vis Absorption (λmax) | ~290-310 nm (β-carboline chromophore, π→π* transition); red-shifted shoulder at ~320-340 nm for 6-OH derivative (n→π* contribution from hydroxyl oxygen lone pair) |
| Fluorescence (λem) | ~440-480 nm (blue-green emission, λex ~340-370 nm); solvent-dependent Stokes shift (larger in polar protic solvents) |
| Solubility | Soluble in DMSO (~10-50 mg/mL), DMF, methanol, and ethanol; limited aqueous solubility (~0.1-1 mg/mL at pH 7); enhanced at alkaline pH (>9) via phenolate formation; formulation with co-solvents or cyclodextrins recommended for biological studies |
| pKa (6-OH) | ~8.5-9.5 (estimated, phenol-like); predominantly neutral at physiological pH 7.4 — favorable for passive membrane permeability |
| Water Content (Karl Fischer) | ≤1.0% |
| Loss on Drying | ≤1.0% (105°C, 2 hours) |
| Ash Content | ≤0.1% |
| Heavy Metals | Total ≤10 ppm; Pb ≤2.0 ppm; As ≤2.0 ppm; Cd ≤1.0 ppm; Hg ≤0.1 ppm |
| Total Plate Count | ≤100 CFU/g |
| Yeast & Mold | ≤10 CFU/g |
| E. coli / Salmonella / S. aureus | Absent in 1 g |
| Residual Solvents (ICH Q3C) | Complies with ICH Q3C limits; Class 2 and Class 3 solvents controlled to ≤0.5% individually by GC-HS |
| Recommended Storage | Store at -20°C ± 5°C, tightly sealed in amber glass vial, protected from light and moisture, under inert atmosphere (argon). Allow vial to equilibrate to room temperature before opening. Protect from prolonged exposure to UV-visible light — β-carboline chromophore is susceptible to photodegradation. Prepare stock solutions in anhydrous DMSO and aliquot into single-use portions. |
| Grade | Research Grade (RUO — Research Use Only) |
| Certifications | ISO 9001:2015, c-GMP |
| Packaging | 10 mg / 25 mg / 50 mg / 100 mg / 250 mg / 500 mg / 1 g in amber glass vials under argon atmosphere; custom packaging available upon request |
| Shelf Life | 2 years from date of manufacture when stored under recommended conditions; retest date on CoA |
Key Benefits — 6-Hydroxycarboline Derivative
β-Carboline Privileged Scaffold — One Framework, Diverse Pharmacological Targets
The β-carboline core is the definition of a privileged medicinal chemistry scaffold. From a single tricyclic framework, appropriately substituted β-carbolines yield high-affinity ligands for MAO-A/MAO-B, 5-HT₂A/5-HT₂C serotonin receptors, benzodiazepine/GABA-A receptors, cyclin-dependent kinases (CDK1/2/5), IKK, topoisomerases, and imidazoline receptors. This extraordinary target diversity — spanning neurological, psychiatric, oncological, and anti-infective indications — arises from the scaffold’s planar aromatic surface (supporting π-π stacking and DNA intercalation), hydrogen-bond-capable nitrogen atoms (N2 pyridine, N9 indole), and well-defined vector geometry for substituent presentation. The 6-OH group extends this target profile by adding a phenolic antioxidant pharmacophore and an additional H-bond donor/acceptor for enhanced target affinity.
Privileged Scaffold6-OH Group — Antioxidant Pharmacophore + Synthetic Derivatization Handle
The hydroxyl at position 6 serves dual roles: (1) Antioxidant activity — the 6-OH donates a hydrogen atom to reactive oxygen/nitrogen species (peroxyl radicals, hydroxyl radical, peroxynitrite), forming a resonance-stabilized phenoxyl radical delocalized across the indole π-system. This radical-scavenging mechanism is the same that underlies the antioxidant activity of phenolic natural products (flavonoids, tocopherols, hydroxytyrosol) and provides neuroprotective, cardioprotective, and anti-inflammatory benefits relevant to the disease indications of β-carboline-based drug candidates. (2) Synthetic versatility — the 6-OH is a chemical handle for O-alkylation (fluorescent tags, PEG linkers), O-acylation (ester prodrugs, electrophilic warheads for covalent inhibitors), conversion to the triflate for Pd-catalyzed cross-coupling (Suzuki, Sonogashira, Buchwald-Hartwig), and O-sulfation/O-phosphorylation (water-soluble prodrugs). This enables rapid diversification of the 6-position for focused library synthesis and SAR exploration without de novo scaffold construction.
Dual-Function 6-OHInherent Fluorescence — Built-in Spectroscopic Reporter for Probe Development
The extended π-conjugation of the β-carboline system produces intense blue-green fluorescence (λem ~440-480 nm) with a large Stokes shift (~70-110 nm) — properties that are tunable through substitution at the 6-position and other sites. The 6-OH group, through its electron-donating mesomeric effect, red-shifts and intensifies the emission relative to the unsubstituted β-carboline. This native fluorescence makes 6-hydroxycarboline derivatives ideal scaffolds for developing: (a) fluorescent probes for monitoring cellular uptake, subcellular localization, and target engagement by fluorescence microscopy, (b) environment-sensitive (solvatochromic) reporters — the emission maximum and quantum yield are sensitive to solvent polarity, pH, and local protein environment, enabling label-free binding assays, and (c) Förster resonance energy transfer (FRET) donors — the blue-green emission overlaps well with common FRET acceptors (fluorescein, rhodamine, Cy3). For biological researchers, this means that the compound can often be tracked directly in cells and tissues without the need for additional fluorescent labeling.
Fluorescent ProbeNatural Product Inspiration — Privileged Scaffold Validated by Millions of Years of Evolution
The β-carboline scaffold is not an arbitrary synthetic construct — it is one of the most widespread and evolutionarily conserved alkaloid frameworks in nature, found across the plant kingdom (Peganum harmala, Banisteriopsis caapi, Passiflora species), marine organisms (Eudistoma genus of ascidians), fungi (Penicillium, Aspergillus), and as endogenous metabolites in mammals (including humans, where β-carbolines are formed by the Pictet-Spengler condensation of tryptamine/tryptophan with aldehydes or α-keto acids). This evolutionary conservation across kingdoms — from plant chemical defense to mammalian neuromodulation — provides compelling validation of the scaffold’s biological relevance and drug-likeness. The 6-hydroxy derivatives specifically have been identified in traditional medicinal plants used for their antioxidant, anti-inflammatory, and neuroprotective properties, providing ethnopharmacological validation that complements the modern medicinal chemistry evidence base. For drug discovery programs: a scaffold that nature has independently evolved for diverse biological functions across multiple phyla carries a significantly higher probability of successful translation than a purely synthetic scaffold with no natural precedent.
Nature-ValidatedApplications
Medicinal Chemistry & CNS Drug Discovery
Starting scaffold for synthesizing focused β-carboline libraries targeting MAO-A/MAO-B (antidepressant, neuroprotective), 5-HT₂A/5-HT₂C (anxiolytic, antipsychotic), GABA-A (anxiolytic, anticonvulsant), and CDK (anticancer) drug candidates. The 6-OH position enables rapid diversification via O-alkylation, O-acylation, or Pd-catalyzed cross-coupling of the corresponding triflate. Compatible with parallel synthesis and fragment-based drug discovery approaches.
Natural Product Total Synthesis & Alkaloid Research
Key intermediate for constructing eudistomin-class marine alkaloids (potent antiviral and anticancer β-carbolines from Eudistoma ascidians), harmala alkaloid analogs (harmine, harmaline, harmol — the 6-O-desmethyl analog), and synthetic β-carboline natural product hybrids. The 6-OH provides a regioselective functionalization point that maps onto the natural 6-oxygenation pattern of many bioactive β-carboline alkaloids.
Neuroscience & MAO Enzyme Pharmacology
Pharmacological probe for studying MAO-A vs. MAO-B isoform selectivity, reversible vs. irreversible MAO inhibition kinetics, and the role of endogenous β-carbolines (tribulin, norharman, harman) in stress, anxiety, depression, and alcohol dependence. 6-OH substitution generally enhances MAO-A inhibitory potency through improved H-bonding with the active-site tyrosine residues (Y407, Y444 in MAO-A).
Fluorescent Probe & Sensor Development
The native blue-green fluorescence of β-carbolines (λem ~440-480 nm, large Stokes shift) makes 6-hydroxycarboline derivatives ideal scaffolds for developing fluorescent sensors, solvatochromic probes, FRET donors, and fluorescent ligands for receptor binding assays. The 6-OH can be derivatized with targeting groups, enzyme-cleavable moieties, or environment-sensitive reporters without fully quenching the chromophore.
Heterocyclic Chemistry & Cross-Coupling Methodology
Versatile building block for transition-metal-catalyzed cross-coupling methodology development. The 6-OH is converted to the corresponding triflate (Tf₂O, pyridine) for Suzuki-Miyaura (aryl/heteroaryl boronic acids), Sonogashira (terminal alkynes), and Buchwald-Hartwig (amines) coupling at the 6-position. Also suitable for studying directing-group effects in C-H activation chemistry on the β-carboline scaffold.
Antioxidant & Redox Biology Research
Chemical probe for studying phenolic antioxidant mechanisms — H-atom transfer (HAT) vs. single-electron transfer (SET) vs. sequential proton-loss electron-transfer (SPLET) — using the 6-OH group as the radical-scavenging pharmacophore. Compare antioxidant capacity (ORAC, FRAP, DPPH, ABTS assays) with other phenolic natural products and synthetic antioxidants to establish structure-activity relationships for the β-carboline scaffold.
Frequently Asked Questions
A 6-Hydroxycarboline Derivative is a functionalized analog of the β-carboline (9H-pyrido[3,4-b]indole) heterocyclic scaffold bearing a hydroxyl (-OH) substituent at the 6-position of the indole ring. The β-carboline core is a tricyclic aromatic system formed by the fusion of a pyridine ring (C-ring) to an indole (A+B rings) — structurally, it is a pyridoindole, isoelectronic with anthracene, and represents one of the most important privileged scaffolds in medicinal chemistry and natural product alkaloid biosynthesis. The 6-hydroxy substitution introduces a hydrogen-bond donor/acceptor at a position that is electronically conjugated with the indole nitrogen, modulating the electron density distribution of the aromatic system and providing a synthetic handle for further derivatization (etherification, esterification, sulfonation, or metal-catalyzed cross-coupling after conversion to the corresponding triflate). β-Carboline alkaloids — including harmine, harmaline, harman, norharman, and the eudistomins — are among the most widespread and pharmacologically significant classes of indole alkaloids in nature, found in plants (Peganum harmala, Banisteriopsis caapi), marine organisms (Eudistoma species), and as endogenous mammalian metabolites. The β-carboline scaffold engages diverse biological targets including monoamine oxidase (MAO-A/MAO-B), serotonin receptors (5-HT₂A, 5-HT₂C), benzodiazepine receptors (GABA-A), cyclin-dependent kinases (CDKs), and DNA (intercalation). 6-Hydroxycarboline derivatives specifically have been investigated for their antioxidant, neuroprotective, and antimicrobial activities, with the 6-OH group contributing to radical-scavenging capacity and to target-binding hydrogen bond networks.
6-Hydroxycarboline derivatives serve as versatile research tools and building blocks across multiple areas: (1) Medicinal Chemistry — the β-carboline scaffold is a privileged structure for drug discovery. 6-Hydroxycarboline derivatives have been explored as leads for neuropsychiatric disorders (MAO inhibitors for depression and Parkinson’s disease, serotonin receptor modulators for anxiety), oncology (CDK inhibitors, DNA intercalators, topoisomerase inhibitors), and infectious diseases (antimalarial, antileishmanial, and antibacterial β-carbolines). (2) Natural Product Total Synthesis — 6-hydroxy-β-carboline is the core structure of numerous bioactive natural products. The 6-OH group serves as a key synthetic handle for installing the complex substitution patterns found in eudistomin-class marine alkaloids, harmala alkaloid derivatives, and synthetic β-carboline libraries. (3) Neuroscience Research — β-Carbolines are endogenous modulators of monoamine neurotransmission. 6-Hydroxycarboline derivatives are used as pharmacological probes for studying MAO enzyme kinetics, serotonin receptor subtype pharmacology, and the role of endogenous β-carbolines (tribulin, norharman, harman) in stress, anxiety, and alcohol dependence. (4) Fluorescent Probe Development — the extended π-conjugation of the β-carboline chromophore produces strong blue-green fluorescence (λem ~440-480 nm) with large Stokes shifts, making 6-hydroxycarboline derivatives attractive scaffolds for developing fluorescent sensors, imaging agents, and environment-sensitive probes. (5) Antioxidant and Redox Biology — the 6-OH group confers radical-scavenging activity via the phenolic antioxidant mechanism. (6) Metal Chelation and Bioinorganic Chemistry — the 6-OH group can form bidentate or tridentate metal-binding sites, making 6-hydroxycarboline derivatives useful ligands for studying metal-β-carboline interactions implicated in neurodegenerative disease.
The 6-hydroxy group profoundly influences both the chemical reactivity and biological profile of the β-carboline scaffold through electronic, steric, and hydrogen-bonding effects: (1) Electronic Effects — the 6-OH is an electron-donating group (+M mesomeric effect) that increases the electron density of the indole ring, activating the 5- and 7-positions toward electrophilic aromatic substitution and lowering the oxidation potential of the aromatic system (making it a better electron donor and radical scavenger). The OH group participates in the extended π-conjugation of the indole, slightly red-shifting the UV-Vis absorption and fluorescence emission spectra. (2) Hydrogen-Bond Donor/Acceptor — the 6-OH serves as both a hydrogen-bond donor (to carbonyl oxygens, carboxylates, and water molecules in protein binding sites) and acceptor (from backbone NH and side-chain OH/NH₂ groups). This dual H-bond capability increases target-binding affinity and specificity compared to the unsubstituted β-carboline, as demonstrated by the ~5-50 fold increase in MAO-A inhibition potency of 6-hydroxy-β-carboline vs. norharman. (3) Synthetic Versatility — the 6-OH is a chemical handle for O-alkylation to install alkyl, benzyl, or fluorescent tags; O-acylation to form ester prodrugs or introduce electrophilic warheads; conversion to the triflate for Pd-catalyzed cross-coupling; and O-sulfation/O-phosphorylation to generate water-soluble prodrugs. (4) Metabolic Stability — the 6-OH is a potential site for Phase II metabolism (glucuronidation, sulfation). This metabolic soft spot can be exploited for prodrug strategies or blocked by steric shielding. (5) pKa and Ionization State — the 6-OH of a β-carboline has an estimated pKa of ~8.5-9.5, meaning it is predominantly unionized at physiological pH 7.4, favorable for membrane permeability while still capable of forming hydrogen bonds.
6-Hydroxycarboline derivatives should be stored at -20°C ± 5°C in tightly sealed, light-protected amber vials under an inert atmosphere (argon or nitrogen). The β-carboline chromophore is susceptible to photodegradation upon prolonged exposure to UV-visible light, and the 6-OH group renders the compound susceptible to slow air oxidation (forming colored quinonoid degradation products). Under recommended storage conditions, the compound is stable for at least 2 years. Solubility: 6-Hydroxycarboline derivatives are typically soluble in DMSO (~10-50 mg/mL), DMF, methanol, and ethanol. Solubility in water is generally low (~0.1-1 mg/mL at pH 7) due to the extended aromatic ring system, but can be enhanced at alkaline pH (>9) through deprotonation of the 6-OH (phenolate formation). For biological assays, prepare 10-100 mM DMSO stock solutions and dilute into assay buffer or culture medium immediately before use (final DMSO concentration ≤0.1% v/v). For in vivo studies, formulation may require co-solvents (e.g., 5% DMSO + 5% Cremophor EL + 90% saline) or cyclodextrin complexation. Handling: Use standard laboratory personal protective equipment (nitrile gloves, safety goggles, lab coat). Work in a well-ventilated area or chemical fume hood. Avoid inhalation of powder, ingestion, and skin/eye contact. In case of skin contact, wash thoroughly with soap and water. Waste disposal should follow institutional guidelines for chemical laboratory waste. A Certificate of Analysis (CoA) and Material Safety Data Sheet (MSDS/SDS) are provided with every shipment.
UPOR Biotech supplies 6-Hydroxycarboline Derivative at Research Grade with a minimum HPLC purity of ≥98.0%, accompanied by a comprehensive Certificate of Analysis (CoA) detailing: assay (HPLC-UV, ≥98.0%), identity confirmation (¹H-NMR — characteristic indole NH at δ 11.0-12.0 ppm, aromatic protons H1/H3/H4/H5/H7/H8 in the δ 7.0-9.0 ppm region, 6-OH broad singlet at δ 9.0-10.5 ppm; ESI-MS — [M+H]⁺ consistent with expected molecular weight; FT-IR — characteristic O-H stretch ~3200-3500 cm⁻¹, indole N-H stretch ~3400 cm⁻¹, aromatic C=C stretches ~1600-1450 cm⁻¹), melting point, water content (Karl Fischer, ≤1.0%), residual solvents (ICH Q3C compliant, GC-HS), heavy metals panel (total ≤10 ppm; Pb ≤2.0 ppm; As ≤2.0 ppm; Cd ≤1.0 ppm; Hg ≤0.1 ppm), and microbiological limits (TPC ≤100 CFU/g, yeast & mold ≤10 CFU/g, E. coli/Salmonella/S. aureus absent). Additional documentation includes: Material Safety Data Sheet (MSDS/SDS), HPLC Chromatogram, ¹H/¹³C NMR Spectra, Mass Spectrum, FT-IR Spectrum, Stability Data (real-time and accelerated), and Complete Lot Traceability. Our manufacturing operations are ISO 9001:2015 certified and conducted under c-GMP guidelines. Standard packaging is amber glass vials under argon atmosphere. All documents are available in English, with translations upon request. Custom packaging, larger quantities, and additional analytical characterization (2D NMR, HRMS, elemental analysis, XRPD) are available upon request. For researchers requiring GLP-compliant analytical support, we can provide characterized reference standards and impurity markers under a separate technical services agreement.